Alex Pines

Alex Pines received his Ph.D. in Biochemistry in 2005 from the University of Trieste, Italy. He is a member of the Department of Molecular Genetics, Erasmus Medical Centre, Rotterdam, The Netherlands, since 2015. His research focuses on elucidating the Transcription-coupled Nucleotide Excision Repair (TC-NER) mechanisms and associated diseases. The deciphering of TC-NER mechanism of action to counteract DNA damage-induced cancer and aging is based on a multi-disciplinary approach that involves proteomics, live imaging and functional genomics.

Recent publications

Sampadi B, Pines A, Munk S, Mišovic B, de Groot AJ, van de Water B, Olsen JV, Mullenders LHF, Vrieling H (2020) Quantitative phosphoproteomics to unravel the cellular response to chemical stressors with different modes of action. Arch Toxicol.  doi: 10.1007/s00204-020-02712-7 PDF

Liebelt F, Schimmel J, Verlaan-de Vries M, Klemann E, van Royen ME, van der Weegen Y, Luijsterburg MS, Mullenders LH, Pines A, Vermeulen W, Vertegaal ACO (2019) Transcription-coupled nucleotide excision repair is coordinated by ubiquitin and SUMO in response to ultraviolet irradiation. Nucleic Acids Research. 48:231-2487 PDF

Sabatella M, Pines A, Slyskova J, Vermeulen W, Lans H (2019) ERCC1-XPF targeting to psoralen-DNA crosslinks depends on XPA and FANCD2. Cell Mol Life Sci. doi: 10.1007/s00018-019-03264-5. PDF

Wienholz F, Zhou D, Turkyilmaz Y, Schwertman P, Tresini M, Pines A, van Toorn M, Bezstarosti K, Demmers JAA, Marteijn JA (2019) FACT subunit Spt16 controls UVSSA recruitment to lesion-stalled RNA Pol II and stimulates TC-NER. Nucleic Acids Res 47:4011-4025 PDF

Pines A, Dijk M, Makowski M, Meulenbroek EM, Vrouwe MG, van der Weegen Y, Baltissen M, French PJ, van Royen ME, Luijsterburg MS, Mullenders LH, Vermeulen M, Vermeulen W, Pannu NS, van Attikum H (2018) TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nat Commun 9:1040. PDF

Evans MD, Mistry V, Singh R, Gackowski D, Różalski R, Siomek-Gorecka A, Phillips DH, Zuo J, Mullenders L, Pines A, Nakabeppu Y, Sakumi K, Sekiguchi M, Tsuzuki T, Bignami M, Oliński R, Cooke MS (2016) Nucleotide excision repair of oxidised genomic DNA is not a source of urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine. Free Radic Biol Med 99:385-391.

Luijsterburg MS, de Krijger I, Wiegant WW, Shah RG, Smeenk G, de Groot AJL, Pines A, Vertegaal ACO, Jacobs JJL, Shah GM, van Attikum H (2016) PARP1 Links CHD2-Mediated Chromatin Expansion and H3.3 Deposition to DNA Repair by Non-homologous End-Joining. Mol Cell 61:547-562.

von Stechow L, Typas D, Carreras Puigvert J, Oort L, Siddappa R, Pines A, Vrieling H, van de Water B, Mullenders LH, Danen EH (2015) The E3 ubiquitin ligase ARIH1 protects against genotoxic stress by initiating a 4EHP-mediated mRNA translation arrest. Mol Cell Biol 35:1254-68.

Dijk M, Typas D, Mullenders L, Pines A (2014) Insight in the multilevel regulation of NER. Exp Cell Res 329:116-23.